Testing clinical samples such as urine, blood or sputum for microbial contaminates is very important to determining the types of infection a patient is suffering. Therefore, it is vital to be able to count bacteria on spiral, spread, settle and pour plates rapidly in order to treat patient effectively and this is where automated colony counting can help.
In clinical laboratories, a more common test for antibiotic susceptibility is a disk diffusion test . The Colonfast zone reader provides automated reading, interpreting, and Expert review of CLSI antibiotic disk tests.
Application of related products
Viable Plate Count [pour plate, spread plate]
Membrane Filter Count
Blood agar Plate Count
Color Colony Counting
Kleihauer-Betke (KB) test/Antibiotic Susceptibility Testing
Viable Plate Count [pour plate, spread plate]
Viable Plate Count is one of the most common methods for enumeration of bacteria. It is assumed that each colony arises from an individual viable cell.
Many biological procedures depend on an accurate count of the bacterial colonies and other organisms. The enumeration of such colonies is a slow, tedious task. When counts are made by more than one technician, wide variations are often noted. Various attempts have been made to speed up the process and improve counting precision.
Colonfast colony counter counts bacterial, yeast, and mould colonies and virus plaque assays automatically on agar plates with color digital imaging technology. Entire plates or selected areas are instantly counted with discrimination by size range and/or color. High resolution color digital imaging better discriminating colonies. True color enables counting mixed cultures and discriminates colonies from debris. Lighting above and below (darkfield or brightfield) the samples provides better contrast and more accurate reading.
Membrane Filter Count
Microbial populations in some natural enviroment are so low that it is sometimes necessary to concentrate them before counting. Large volumes (one liter) are passed through a membrane filter imprinted with grid pattern. The filter can be stained and cut into strips that will fit on a microscope slide. When immersion oil is added to the filter slide preparation, the filter becomes transparent leaving only stained bacteria and the grid for examination under oil immersion lens of a compound microscope, e.g.for water analysis.
Blood agar Plate Count
BAP contains mammalian blood (usually sheep or horse), typically at a concentration of 5¨C10%. BAP are an enriched, differential media used to isolate fastidious organisms and detect hemolytic activity. ¦Â-hemolytic activity will show complete lysis of red blood cells surrounding colony.
Color colony count
A firm and secure colour evaluation is of particular importance if chromogene nutrient-agar gives a specific colouring to the varying types of bacteria. Using the effective COLONFAST image analyzer the differently coloured colonies are reproducibly classified and counted.
Kleihauer-Betke (KB) test/Antibiotic Susceptibility Testing
In clinical laboratories, a more common test for antibiotic susceptibility is a disk diffusion test . In this test the bacterial isolate is inoculated uniformly onto the surface of an agar plate. A filter disk impregnated with a standard amount of an antibiotic is applied to the surface of the plate and the antibiotic is allowed to diffuse into the adjacent medium.. The result is a gradient of antibiotic surrounding the disk. Following incubation, a bacterial lawn appears on the plate. Zones of inhibition of bacterial growth may be present around the antibiotic disk. The test is performed under standardized conditions and standard zones of inhibition have been established for each antibiotic. If the zone of inhibition is equal to or greater than the standard, the organism is considered to be sensitive to the antibiotic. If the zone of inhibition is less than the standard, the organism is considered to be resistant.
The Colonfast zone reader facilitates accurate, fully quantitative susceptibility testing in clinical microbiology laboratories. It provides automated reading, interpreting, and Expert review of CLSI antibiotic disk tests.
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